1. Analysis of the bacterial microflora of Apis mellifera carpatica in Romania
1.1. collection of bee samples from Romanian apiaries.
1.2. isolation and culture of predominant honeybee gut bacteria.
1.3. evaluation of the probiotic bacteria from the honeybee gut.
2. Creation of a new probiotic product formula
2.1. Establishing the optimal dose of probiotic bacteria for the innovative product.
2.2. Microencapsulation of beneficial bacteria with the addition of B vitamin complex.
3. Definition of bees batches and treatment application
3.1. Realization of artificial infestation with viable spores of Nosema and application of treatment with the new probiotic product.
3.2. Comparison and statistical analysis of host prevalence of Nosema spp.
3.3. Evaluation of the development of bee colonies in Romanian apiaries after the application of treatment with the new probiotic product.